Teacher: Surey Rios

Topic: Paper Chromatography

Subject: Comprehensive Science

Paper Chromatography

Behavioral Objective:

The student, after an introduction to chromatography, will be able to use basic chromatography techniques to solve a crime and write a four paragraph essay on their findings using 4 key terms.

Content Outline:

Chromatography

1. Definition
2. History
3. Chromatography Today

Drugs

DNA

Arson

Gun Powder

1. Key Terms

Rf values, calculating

Solvent

Solvent front

Dissolve

"Like substances"

Traveling

Instructional Strategies

SET- The teacher will play the soundtrack to the theme "Hawaii 5-0" in the background as the teacher prepares the class. The teacher will then start the class by telling them a story of a crime (see attached page) and showing them the evidence that was left on the scene. The teacher will then show the class how each piece of evidence was evaluated by using different separation techniques. The teacher will then tell the class that they too have their own crimes to solve using a technique called paper chromatography. The teacher will then ask the students if they have ever seen ink run off from a piece of paper and having it smear. Some/All students will answer "yes." The teacher will then say that that is a paper chromatography in a simplified form.

BODY- Effective Explanation: Using the sequence and scope of the material outlined in the Content Outline of the plan, the teacher will further clarify the following using overhead transparencies and a laboratory activity. (See attached lab activity)

Basic techniques in a paper chromatograph

Calculating Rf values

Key terms

VISUAL	AUDITORY	KINESTHETIC
Chromatography lab Transparencies of different separation techniques	Hawaii 5-0 soundtrack Lecture	Chromatography lab activity

Assessment: The students will be given two forms of assessment.

1. Group Lab Report which must include

Cover page

Hypothesis

Problem statement

Materials

Procedures

Observations

Data

Calculations

Conclusion

1. Four Paragraph Essay with 4 key terms

Chromatography

Solvent

Rf values

Dissolve

"Like substances"

Traveling

Materials-

Standard/Unknown of Ink Chromatography (1 for each group)

Coffee filters

Scissors

Felt tip pens (different colors)

Water/Alcohol

Medium sized glass jars

Tape

Ruler

Pencils

Who Done It worksheets (1 for each group)

Suspect Cards (1 set for each group)

White paper

Hawaii 5-0 theme music

Pictures of other forms of chromatography

Evaluation: The students will be given 2 grades, one group grade for the group lab activity, and an individual grade for the four paragraph essay. The grades will be calculated using the following rubric:

Assignment	A	B	C	D
Group Lab Report	Above satisfactory completion of all 9 parts and 90% of the calculations correct	Above satisfactory completion of 7 parts and 80% of the calculations correct	Satisfactory completion of 5 parts plus 70% of the calculations correct; missing some data	Satisfactory completion of 4 parts, 50% off the calculations correct, a lot of data missing; incorrect results or conclusion
Individual Essay	Neatly typed/handwritten with use of proper grammar and spell checked. Uses 4 or more key terms. Applies key terms to their observations and results	Neatly typed/handwritten with use of proper grammar and spell checked. Uses 4 key terms but does not apply them to the lab assignment	Neatly typed/handwritten. Has some grammar and spelling errors. Uses 3 key terms and does not apply them to the lab assignment.	Handwritten with poor grammar and spelling errors. Uses two key terms and does not apply them to the lab assignment.

References:

Almirall, Jose R, Ph.D.. "Separation Science." Lecture on Chromatography. Florida International University. Miami, Florida. May 27, 2000.

Dade County Public Schools. Content Based Curriculum: M/J Comprehensive Science 1-3.

Florida Department of Education. Sunshine State Standards: Grades 6-8. [Online] http://www.firn.edu/doe

Houde, John. Crime Lab: A Guide for Nonscientists. Ventura: Calico Press. 1999.