Take Home Quiz 1.  Be sure to show your calculations in the space provided.

1.  How many microliters are in 0.457 ml?  (1 point)






2.  Suppose you had 85µl of a protein sample.  Of this 12 µl were diluted into 108 µl of water.  Of this dilution
25 µl were submitted to Bradford protein assay and produced an OD 0.63.   This is a thought question:  for pure
molecules, one would use the Lambert-Beer equation and need the extinction coefficient.  But, some biochemical
assays do not contain pure molecules under defined conditions, thereby require a standard curve.  If you have
been doing this lab, you already have a prior standard curve, where?
    a.  What is the dilution from the original sample that 25 µl measured in Bradford assay represents?  (2 points)









    b.  How much protein is in the original protein sample?  (3 points)











    c.  How much protein is left in the original sample at the end of the expirment?  (2 points)









3.  Suppose you had 23 µl of a DNA sample that had 45 nmoles DNA/ml.  How much would you need to
dilute the sample to get 4 pmoles/ml?    (2 points)