PCB 5215

Histochemistry & Immunocytochemistry Workshop


Nerve terminal in embryonic day 20 rat labeled with anti-synaptophysin antibody


Fall Semester 2000Class time: ARRClass room:    OE 251
Instructor:    K. Condon, Ph.D.Office: OE 244phone: x2604 e-mail: condon@fiu.edu


SessionTopicReading Assignment
week 1 Tissue preparation Ch. 1-5 and selected readings
week 2 Paraffin sectioning & staining Ch. 6-11
week 3 Cryotomy Ch. 23 and selected readings
week 4 Histochemistry Ch. 23 and selected readings
week 5 Immunocytochemistry Ch. 24 and selected readings

Text: Presnell, JK, MP Schreibman and GL Humason (1997) Humanson's Animal Tissue Techniques, 5th ed. Baltimore: Johns Hopkins University Press. ISBN: 0-8018-5401-6.
Grading:P/F based on a submitted slide set and report documenting the required techniques.


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