KALAI MATHEE, MSc, PhD.

Founding Chair & Associate Professor

Department of Molecular Microbiology and Infectious Diseases

College of Medicine, Florida International University, Miami, USA

www.fiu.edu/~matheek

ORAL PRESENTATIONS

Invited Lectures
No	Date	Venue, Date, Title
47-8	10/2009	Two lectures: Mount Sinai Hospital, Miami Beach, Florida. Oct 2^nd: Mutations and Repair Systems; Oct 4^th: Polymerase Chain Reaction
46	09/2009	Mount Sinai Hospital, Miami Beach, Florida. Aug 24^th. DNA Replication
45	08/2009	Mount Sinai Hospital, Miami Beach, Florida. Aug 24^th. The Chemical Nature of the Gene
44	06/2009	Grand Rounds at Mount Sinai Hospital, Miami Beach, Florida. June 19^th. Pseudomonas aeruginosa: How do we combat chronic Infections in cystic fibrosis?
43	03/2009	Lunch Seminar, Division of Pulmonary and Critical Care Medicine, University of Miami, FL. March 4^th. Molecular genetic studies of Pseudomonas aeruginosa pathogenicity.
42	11/2008	St. Lucie – Okeechobee County Medical Society Annual Education conference. Nov 8^th. Vaccination
41	10/2008	Wright Symposium. Jackson Laboratories, Oct 1^st – 5^th. Bangor, Maine. Pseudomonas aeruginosa and cystic fibrosis
40	05/2008	Mount Sinai Hospital, Miami Beach, Florida. May 23^rd: Polymerase Chain Reaction
36-9	04/2008	Mount Sinai Hospital, Miami Beach, Florida. April 4^th: Chromosome Structure and The Chemical Nature of the Gene April 18^th: DNA Replication April 25^th:Mutations and Repair Systems
35	03/2008	9^th Comparative Immunology Symposium. Immunodiversity: Cellular and molecular interactions. Miami, FL. Mar 6^th – 7^th. Dynamics of Pseudomonas aeruginosa genome evolution.
34	01/2008	10^th Asian-Pacific Conference on Transcription (ACT-X), Jan 13^th – 16^th, Bangalore, India. Regions of genome plasticity in Pseudomonas aeruginosa revealed by comparative genomics.
33	12/2007	Postgraduate Institute of Medical Education and Research, Chandigarh, India. Potential application of microbial metagenome profiling using amplicon length heterogeneity-polymerase chain reaction (ALH-PCR) for polymicrobial diseases.
32	03/2007	8^th Biomedical and Comparative Immunology Symposium. Immunodiversity: Cellular and molecular interactions. The ability of the South Florida medicinal plants to inhibit bacterial virulence through interruption of cell-cell communication.
31	02/2007	Pseudomonas aeruginosa amp genes and ß-lactam resistance. Lory Lab Meeting, Harvard Medical School
30	11/2006	1^st First International Conference on Forensic Science, Costa Rica. Potential application of microbial metagenome profiling using amplicon length heterogeneity-polymerase chain reaction (ALH-PCR) for crime scene investigations.
29	09/2006	Pseudomonas aeruginosa algO encodes a putative periplasmic protease that is a positive regulator of alginate production, Lory Lab Meeting, Harvard Medical School
28	09/2006	MGH Monthly Pathogenesis Club, Department of Molecular Biology. MGH, Boston. Pseudomonas aeruginosa algO encodes a putative periplasmic protease that is a positive regulator of alginate production.
27	12/2005	9^th Asian Conference on Transcription (ACT-IX), Taipei, Taiwan. Prc(tsp), a novel protease involved in the regulation of Pseudomonas aeruginosa extracytoplasmic function sigma factor AlgT/U.
26	07/2005	Second Annual McNair Post Baccalaureate Achievement Program 2005 Summer Research Institute Closing Ceremony, FIU. How does one measure the success of the McNair Program?
25	03/2005	7^th Comparative Immunology Symposium. Immunodiversity: Cellular and molecular interactions. Pseudomonas aeruginosa AmpR is a global transcriptional factor that regulates expression of AmpC and PoxB b-lactamases, proteases, quorum sensing and other virulence factors.
24	02/2005	Annual FIU College of Science’s External Advisory Board Meeting, Colonnade Hotel, Coral Gables, Miami Florida. Biomedical and forensic research training: The role of students.
23	02/2004	Forensic Science Symposium 2004, Ft Lauderdale, FL (2/27 to 2/28). Nonhuman DNA: Application of microbial community analysis for crime scene investigations.
22	03/2004	FIU Department of Biological Sciences Seminar Series. Nonhuman DNA: Application of microbial community analysis for crime scene investigations.
21	05/2004	80^th Annual American Chemical Society Meeting – FAME 2004. Orlando, FL. May 6^th to 8^th. Mathee, K., D. Mills, S. Miller, S. King, J. Entry, and G. Narasimhan. Microbial forensics: Application of microbial community metagenome analysis for crime scene investigations. Abstract #68
20	11/2002	Dietetics and Nutrition Seminar Series, FIU. Ginseng to the rescue of cystic fibrosis patients.
19	08/2002	Malaysia Nurses Association, Penang Branch, Malaysia. Women’s challenges in the new millennium.
18	08/2002	Institute of Biological Sciences (Genetics), University of Malaya, Kuala Lumpur, Malaysia. Two lectures: (1) Microbial cell-to-cell communication and search for interrupters. (2) Molecular Identification of Microbes using the 16S rRNA.
17	04/2002	Prokaryotic Interest Group, Florida International University. Molecular identification of microbes using the 16S rRNA.
16	03/2002	Liberal Studies Colloquium, Florida International University. Plagues in medicine and myth.
15	12/2001	Gangagen Biotechnologies PVT, Ltd., Bangalore, India. Chronic infection with Pseudomonas aeruginosa: alginate, biofilms, alternate therapy and the host immune response.
14	11/2001	The Dean’s Seminar Series 2001, College of Health Sciences, University of Florida, Gainesville. Role of polymorphonuclear leukocytes and alginate production in the Pseudomonas aeruginosa biofilms in the lungs of cystic fibrosis patients.
13	08/2001	14^th Evergreen International Phage Meeting, Olympia, WA. Potential alternate therapies against Pseudomonas aeruginosa biofilm growth.
12	10/2000	Rotary Club of Kuala Lumpur West at the Royal Selangor Club, Malaysia. Communication between bacteria.
11	05/1999	University of Tennessee-Memphis, College of Medicine, Department of Microbiology and Immunology, Memphis, Tennessee. Mechanism of mucoid conversion in Pseudomonas aeruginosa associated with chronic pulmonary infection in patients with cystic fibrosis.
10	05/1999	Technical University of Denmark, Department of Microbiology, Lyngby, Denmark. Cloning and characterization of the alginate biosynthetic genes of Pseudomonas fluorescens.
9	03/1999	Basic and Clinical Research of Chronic Pseudomonas aeruginosa Lung Infection in Cystic Fibrosis and Diffuse Panbronchiolitis - East meets West. Copenhagen, Denmark. March 17^th – 19^th. Induction of alginate production in vivo.
8	06/1998	The European Cystic Fibrosis Conference Berlin, Germany. In the “Late Breaking Science” session. K. Mathee, O. Ciofu, N. Høiby, D. Ohman, S. Molin and A. Kharazmi. Mucoid conversion of Pseudomonas aeruginosa by activated polymorphonuclear leukocytes: a mechanism for virulence activation in the cystic fibrosis lung.
7	06/1998	K. Mathee, O. Ciofu, N. Høiby, D. Ohman, S. Molin and A. Kharazmi. Molecular mechanism responsible for the conversion of non-mucoid to the fatal mucoid form of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients. Technical University of Denmark, Department of Microbiology, Lyngby, Denmark.
6	11/1997	Centre Medical Universitaire, Departement de Biochimie Medicale, Geneve, Switzerland. Conversion of non-mucoid to the mucoid form of Pseudomonas aeruginosa is associated with chronic pulmonary infection in cystic fibrosis.
5	11/1997	Technical University of Denmark, Department of Microbiology, Lyngby, Denmark. Conversion of non-mucoid to the fatal mucoid form of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients is induced by oxygen radicals.
4	11/1997	University of Copenhagen, Rigshospitalet, Department of Clinical Microbiology, Denmark. Role of hydrogen peroxide in the conversion of non-mucoid to the fatal mucoid form of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients.
3	04/1997	Technical University of Denmark, Department of Microbiology, Lyngby, Denmark. Alginate gene regulation by alternative sigma factor in Pseudomonas aeruginosa.
2	09/1996	1^st ASM Conference on Microbial Biofilms. Snowbird, Utah. The role of an alternative sigma factor in alginate regulation.
1	12/1995	University of Malaya, Faculty of Medicine, Department of Medical Microbiology, Malaysia. Regulation of alginate production.
Oral Presentations at Conferences
No	Date	Authors, Title, Venue
12	09/2007	Annual Meeting of the Florida Branch American Society for Microbiology, Oct 6^th – 8^th, St. Petersburg, FL. K. Mathee, K.F. Kong and A. Adonizio. Use of south Florida medicinal plants to inhibit quorum sensing.
11	12/2005	9^th Asian Conference on Transcription (ACT-IX), Dec 12^th – 15^th, Zhunan Town, Taiwan. R. T. Sautter, and K Mathee. Prc(tsp), a novel protease involved in the regulation of Pseudomonas aeruginosa extracytoplasmic function sigma factor AlgT/U.
10	03/2005	7^th Comparative Immunology Symposium. Immunodiversity: Cellular and molecular interactions. Miami, FL. Mar 4^th – 5^th. K. Mathee, K.F. Kong and S.R. Jayawardena. Pseudomonas aeruginosa AmpR is a global transcriptional factor that regulates expression of AmpC and PoxB b-lactamases, proteases, quorum sensing and other virulence factors.
9	06/2004	27^th European Cystic Fibrosis Conference. June 12^th – 17^th, Birmingham, UK. S. Miller, D. Mills, T. Pressler, C. Koch, and K. Mathee.Fingerprinting of cystic fibrosis sputum metagenome reveals unique amplicon length heterogeneity (ALH) profiles for each patient.
8	06/2001	24^th European Cystic Fibrosis Conference, June 6^th –9^th, Vienna, Austria. A Plata, M. Hentzer,N. Hoiby,S. Molin, A. Kharazmi, and K. Mathee. Activation of alginate genes by polymorphonuclear leukocytes.
7	10/2000	6^th Asian Conference on Transcription (ACT-VI), Oct 23^rd – 27^th, Beijing, China. K. Mathee, M. Hentzer, and O. Ciofu. Inverse relationship between alginate production and other extracellular virulence factors in Pseudomonas aeruginosa is mediated by quorum sensing system.
6	06/2000	13^th International Cystic Fibrosis Congress, June 4^th – 8^th, Stockholm, Sweden. K. Mathee, M. Hentzer, A. Heydorn, N. Høiby, D. Ohman, S. Molin, and A. Kharazmi. Microcolony formation of Pseudomonas aeruginosa in cystic fibrosis lungs is influenced by infiltrating polymorphonuclear leukocytes. Abstract #332.
5	09/1999	2^nd Annual Meeting of the Cold Spring Harbor Laboratory on Microbial Pathogenesis and Host Response, Sept 22^nd – 26^th, Cold Spring Harbor, NY. K. Mathee, M. Hentzer, A. Heydorn, M.Givskov, N. Høiby, D. Ohman, S. Molin, and A. Kharazmi. The structure of Pseudomonas aeruginosa biofilms in lungs of cystic fibrosis patients may be dictated by host immune response.
4	08/1998	The Annual Meeting of the Cold Spring Harbor Laboratories on Molecular Genetics of Bacteria and Phages, Aug 25^th – 30^th, Cold Spring Harbor, NY. K. Mathee, O. Ciofu, C. Sternberg, P. Lindum, J. Campbell, P. Jensen, A.H. Jøhansen, D. Ohman, M. Givskov, S. Molin, N. Høiby, and A. Kharazmi. Conversion to the mucoid form of Pseudomonas aeruginosa, associated with chronic pulmonary infection in cystic fibrosis, can be induced by hydrogen peroxide or activated polymorphonuclear leucocytes. Abstract #165.
3	03/1996	4^th Asian Conference on Transcription (ACT-IV), Mar 30^th – Apr 2^nd, Hayama, Kanagawa, Japan. K. Mathee, and D.E. Ohman. Evidence of post-translational regulation of algT that encodes an alternative sigma factor in Pseudomonas aeruginosa involved in alginate gene regulation. Abstract #57.
2	08/1992	The Annual meeting on the Molecular Genetics of Bacteria and Phages, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. K. Mathee, and M.M. Howe. Mor-dependent activation of the phage Mu middle promoter requires the E. coli s70 subunit and core polymerase. Abstract #111.
1	08/1990	The Annual Meeting on the Molecular Genetics of Bacteria and Phages. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. K. Mathee, and M.M. Howe. Identification of a positive regulator for bacteriophage Mu middle operon transcription. Abstract #89.
Oral Presentations at Conferences by Students/Fellows
No	Date	Authors, Title, Venue
29	04/2009	2009 Graduate Student Association Annual Scholarly Forum ¨ M. Doud, P. Gillivet, M. Light and K. Mathee. Community Sequencing Analysis of Bacterial Communities Found within Cystic Fibrosis Lung.
28	10/2008	The 22nd Annual North American Cystic Fibrosis Conference, Orlando. Florida. October 23rd – 25th. ¨ M. Doud, N. Baker, L. Schneper, M. Light, J. Martinez, and K. Mathee. Molecular analysis showing diverse respiratory fungal infections in south Florida cystic fibrosis patients.
27	03/2008	8th Comparative Immunology Symposium. Immunodiversity: Cellular and molecular interactions. Miami, FL. Mar 6th – 7th. ¨ N. Baker, M. Doud, L. Schenepr and K. Mathee. Fungal analysis of the cystic fibrosis lung using Amplicon Length Hetereogentiy – Polymerase Chain Reaction Melissa received the third prize for the Presentation Award
25 - 26	02/2008	10th Annual Biology Research Symposium, Feb 16th, Kampong Botanical Gardens, Miami, FL ¨ D. Balasubramanian, L. Schneper, O. Caille, M. Merighi, A. Brencic, R. Smith, E. Zeng, S. Lory, G. Narasimhan, K. Mathee; Elucidating the Pseudomonas aeruginosa AmpR regulon. ¨ M.S. Doud, N. Baker, L. Schneper, K. Mathee; Metagenomic analysis of the cystic fibrosis lung using Amplicon Length Heterogeneity Polymerase Chain Reaction. Melissa was placed second in the Best Presentation Award.
24	10/2007	2007 Annual Meeting of the Florida Branch American Society for Microbiology, Oct 6th – 8th, St. Peterburg, FL. ¨ M. Doud and K. Mathee. Molecular analysis cystic fibrosis sputum metagenome using Amplicon Length Heterogeneity – Polymerase Chain Reaction (ALH-PCR) Melissa was placed second in the Best Presentation Award in the Graduate Students Category.
23	09/2007	FIU Minority Biomedical Research Mini-Symposium. University Park. FL. Sept 27th. ¨ M. Doud and K. Mathee. Molecular analysis of the polymicrobial disease, cystic fibrosis, using Amplicon Length Heterogeneity – Polymerase Chain Reaction (ALH-PCR) Melissa won the Best Presentation Award in the Graduate Students Category
22	06/2006	47th Annual Meeting of the Society for Economic Botany in Chiang Mai, Thailand. June 5th-9th, 2006. ¨ A. Adonizio, K.F. Kong, and K. Mathee. Anti-quorum sensing potential of South Florida medicinal plants and the attenuation of Pseudomonas aeruginosa pathogenicity.
21	02/2006	American Academy of Forensic Sciences Annual Meeting. ¨ L. I. Moreno, D. Mills, J. Entry, R. Sautter and K. Mathee. Potential use of Microbial DNA in soil forensics. Lilliana’s abstract was selected publication of the full article at the Journal of Forensic Science
20	03/2006	8th Biomedical and Comparative Immunology Symposium. Immunodiversity: Cellular and molecular interactions. Miami, FL. Mar 3th – 4th. ¨ A. Adonizio, and K. Mathee. Anti-quorum sensing agents from South Florida medicinal plants and their potential to attenuate Pseudomonas aeruginosa pathogenicity. Allison received the second prize for the Presentation Award
19	09/2005	45th Annual International Conference on Antimicrobial Agents and Chemotherapy (ICAAC) on September 21 – 24, 2005 in New Orleans, LA. ¨ K.F. Kong, S.R. Jayawardena, S. Indulaker, A. del Puerto, C. Koh, N. Høiby and K. Mathee. Pseudomonas aeruginosa AmpR is a global transcriptional factor that regulates expression of AmpC and PoxB b-lactamases, proteases, quorum sensing and other virulence factors. Mr. Kong also received a travel award to this meeting. However it was cancelled due to the Hurricane Katrina.
18	07/2005	FIU 2005 Summer Research Institute Symposium. Ronald E. McNair Postbaccalaureate Achievement Program. ¨ K. Anson, G. Tachiev, K. Mathee. Development of mold remediation process using hydrogen peroxide catalyzed by Fe(II) iron. Ms. Anson won the first prize for the Poster Presentation and third prize for the best Research Report.
17	06/2005	46th Annual Meeting of the Society for Economic Botany at Texas Christian University, Ft. Worth, TX June 5th-10th, 2005. ¨ A. Adonizio, K. Downum, B. Bennett, and K. Mathee. Anti-quorum sensing compounds from plants: expanding or view of anti-bacterial efficacy.
16	03/2005	2005 Graduate Student Association Annual Scholarly Forum ¨ M. Doud. S. Miller, D. Mills, and K. Mathee. Profiling of microbial communities using amplicon length heterogeneity-polymerase chain reaction. Ms. Doud won the first prize for oral presentation in the Life Sciences Category.
15	05/2005	81st Annual American Chemical Society Meeting – FAME 2005. Orlando, FL. May 6th to 8th. ¨ L. Moreno, D. Mill, J. Entry, T.T. Sautter and K. Mathee. Molecular and chemical characterization of three Miami-Dade soil types for forensic comparison.
14	06/2004	The Joint 45th Annual Meeting of the Society for Economic Botany with the International Society of Ethnobiology and the 8th International Society for Ethnopharmacology at University of Kent at Canterbury, UK. June 13th – 17th. ¨ A. Adonizio, K. Downum, B. Bennett, and K. Mathee. Identification of quorum sensing inhibitors in South Florida medicinal plants: an understudied aspect of efficacy. Ms. Adonizio received the best presentation award.
13	03/2004	6th Comparative Immunology Symposium. Immunodiversity: Cellular and molecular interactions. Miami, FL. Mar 4th – 5th. ¨ K-F. Kong, S. Jayawardena, A. del Puerto, and K. Mathee. Characterization of poxA, a chromosomal-encoded Pseudomonas aeruginosa oxacillinase.
12	01/2004	2003/2004 Biology Research Symposium, FIU, Miami, FL. Jan 31st. ¨ R. Sautter, D. Ramos, and K. Mathee. Molecular mechanisms involved in the mucoid-to-nonmucoid conversion of Pseudomonas aeruginosa.
11	10/2003	FIU MBRS Mini-Symposium. University Park. FL. Oct 1st. ¨ R. Sautter, and K. Mathee. Molecular mechanisms involved in the mucoid-to-nonmucoid conversion of Pseudomonas aeruginosa.
10	10/2002	FIU MBRS Mini-Symposium. University Park Campus, Miami, FL. Oct 2nd. ¨ A. Aguila, and K. Mathee. Topology analysis of Pseudomonas aeruginosa AmpG, a putative permease.
9	07/2002	7th Asian Conference on Transcription (ACT-VII), Kuala Lumpur, Malaysia. July 23rd – 27th. Ms. Ramos received numerous travel awards from various sources. ¨ G. Narasimhan, C. Bu, Y. Gao, X. Wang, N. Xu, G. Zheng, and K Mathee. Mining protein sequences for motifs. Abstract #7033.
8	02/2002	4th Comparative Immunology Symposium. Miami, FL. Feb 28th – Mar 1st. ¨ Z. Song, H. Wu, A. Kharazmi, N. Høiby, O. Ciofu, J. Rygaard, and K. Mathee. Alginate production protects Pseudomonas aeruginosa from host immune clearance in a mouse model of acute lung infection.
7	02/2002	Florida Georgia-Stokes League in Alliance for Minority Participation Exposition, Orlando, FL. ¨ M. R. Iriarte, and K. Mathee. Bacterial communication in expression of the virulent genes of the pathogenic Pseudomonas aeruginosa. Ms. Iriarte was placed second in Life-Science Presentation Category.
6	06/2000	13th International Cystic Fibrosis Conference. Stockholm, Sweden. June 4th – 8th. ¨ H. Wu, Z. Song, M. Givskov, K. Mathee, J. Rygaard, and N. Høiby. Quorum sensing systems on chronic lung Infection of Pseudomonas aeruginosa in rat model. Abstract #310.
5	09/1997	6th International Congress on Pseudomonas: Molecular Biology and Biotechnology. Madrid, Spain. Sept 4th – 8th. ¨ D. E. Ohman, K. Mathee, S. Ma, and C.J. McPherson. Post-translational control of an alternative sigma factor in the expression of the alginate regulon in Pseudomonas aeruginosa. Abstract #IV. Invited talk.
4	08/1997	The Annual meeting of the Cold Spring Harbor Laboratories on Molecular Genetics of Bacteria and Phages. Madison, Wisconsin. Aug 5th – 10th. ¨ D.E. Ohman, K. Mathee, and C.J. McPherson. Post-translational control of an alternative sigma factor in the expression of the alginate regulon in Pseudomonas aeruginosa. Abstract #56.
3	08/1995	5th International Symposium on Pseudomonas: Molecular Biology and Biotechnology. Tsukuba, Japan. Aug 21st – 25th. ¨ D.E. Ohman, K. Mathee, C. McPherson, S. Ma, and M.J. Franklin. Regulation of the alginate (algD) operon in Pseudomonas aeruginosa. Invited talk.
2	05/1995	95th Annual Meeting of the American Society for Microbiology. Washington, D.C. May 21st– 25th. ¨ D.E. Ohman, C.J. McPherson, and K. Mathee. Transcriptional analysis of algT encoding a putative alternate sigma factor in Pseudomonas aeruginosa involved in alginate gene regulation. Abstract #B77. Invited talk.
1	07/1988	The EMBO Workshop on Gene Organization and Expression in Bacteriophages, Salamanca, Spain, p11. ¨ M.M. Howe, W. Margolin, S.F. Stoddard, K. Mathee, and G. Rao. Regulation of middle and late transcription of bacteriophage Mu. Invited talk.