We are currently working on the development of specialized polymer sieving buffers for rapid DNA separation in microchips. Associated with this work we are working on rapid PCR amplification methods using high speed thermal cylcing
Rapid PCR, microfluidics and DNA separations
PCR Inhibition Studies
Aboud MJ; Gassmann M.; McCord B. The development of mini pentameric STR loci for rapid analysis of forensic DNA samples on a microfluidic system. Electrophoresis 2010 31(15) 2672-9
Boulas, S. ; Blas, M.; Cabrices, O.; McCord, B. Development of an entangled polymer solution for improved resolution in DNA typing by capillary electrophoresis, Electrophoresis, 2008 29(23) 4695-4703..
Opel, K.; Chung, D.;McCord, B. A Study of PCR Inhibition Mechanisms Using Real Time PCR, J. Forensic Sci, 2010, 55(1) 25-33.
Maribel E. Funes-Huacca1, PhD; Kerry Opel2, Ph.D; Bruce McCord1, PhD. A comparison of the effects of PCR inhibition in Quantitative PCR and forensic STR Analysis, Electrophoresis, 2011, 32(9), 1084–1089.
Forensic DNA samples can be affected by coextracted materials (PCR inhibitors) present at a crime scene. Using Multiplex PCR amplification and real time PCR with high resolution melt capability, we are examining a variety of PCR inhibitors with respect to DNA sequence and polymerase binding. We model their effects and work on better ways to reduce inhibition to permit forensic scientists to improve the quality of their data.
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