Cell
culture model systems for cell types and regions in
the nervous system have been very important in allowing
the study of neural processes at the cellular and molecular
levels, and have helped us understand underlying mechanisms
of the vertebrate nervous system, a very complex and
cellularly heterogenous system.
This workshop on culturing primary brain cells is to
guide you progressively through the fundamental steps
necessary for successfully growing and maintaining primary
brain cells.
The workshop is divided into three parts:
1. Theoretically learning how to set-up and equip a
cell culture laboratory; sterilization of fluids &
equipment; culture media; culture technique & primary
culture; prevention, detection & remedies for contamination
and good laboratory practice in the cell culture laboratory.
2. Practically applying techniques learned by preparing
instruments, solutions, equipment, etc., in readiness
for growing and maintaining cultured brain cells.
3. 2-3 person Teams harvesting & maintaining a
selected brain cell type culture.
Credits: 2
Instructor: Ophelia I. Weeks, Ph.D.
Office HLS 219C; Lab HLS 229
Email Address: weekso@fiu.edu
Time & Place: Tissue Culture Core Laboratory (HLS
232)
Required Reference Text: Neural Cell Culture. A Practical
Approach, edited by J. Cohen and G. P. Wilkin, IRL Press
at Oxford University Press, 1995.
Recommended Text: Basic Cell Culture. A Practical Approach,
edited by J.M. Davis, IRL Press at Oxford University
Press, 1994
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